A question about scales

Hello there,
I am designing a 3D wall image and I am wondering something.
I would like to know the rules to get a 1:1 3D to 2D ratio scale on screen, to have my pictures displayed at their “best”.
For example, if I have two vertices like
{x:0,y:0,z:0}
{x:200,y:0,z:0}
Then I would like them to be separated by exactly 200pix from each other …

I am not sure I use the right words to be understood… The goal is that when I have a photo at a z of 0, she would be at her real size.

Any help, links, etc, greatly appreciated.

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